Study Protein-Protein Interactions Within Cells
Pharmacological Intervention to prevent proteins interacting with each other represent a novel approach for drug discovery. This poster describes how two proteins can be labelled such that when these proteins interact, the recombination of the labels results in a luminescence readout that translates into a fluorescence readout. A reduction in fluorescence in the presence of a compound indicates inhibition of the interaction. This interaction can be monitored in living cells allowing a phenotypic, arguably a more physiological readout. In our example Schnurri-3 interacts with ERK-2 to modulate bone density. By pharmacologically manipulating this interaction we could increase bone density for oesteoporetic patients.